Development of a highly sensitive direct microtiter plate ELISA for human serum testosterone
نویسندگان
چکیده
منابع مشابه
DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...
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In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA sho...
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Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELIS...
متن کاملdevelopment of a simple and sensitive enzyme- linked immunosorbent assay (elisa) for clinical measurement of testosterone using penicillinase as label
an enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (t-3-0-cmo-bsa ) and penicillinase-labelled t-3-0-cmo was developed. this assay was utilized to measure testosterone in serum samples of male and female subjects. the sensitivity of the assay is 50pg/well and the antibody developed crossreacted...
متن کاملExpansion of a highly sensitive and specific ELISA test for diagnosis of hydatidosis using recombinant EgB8/2 protein
Objective(s): Hydatidosis is a zoonotic infection and endemic in Iran. Due to the serological cross-reactivity (of sera) with other parasitic infection, diagnosis of hydatid cyst is considered to be problematic. In this regard, application of recombinant antigens improves serological diagnosis for human hydatidosis. Here, we present an ELISA test based on B8/2 recombinant antigen of Echinococcu...
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ژورنال
عنوان ژورنال: Indian Journal of Clinical Biochemistry
سال: 1999
ISSN: 0970-1915,0974-0422
DOI: 10.1007/bf02869147